Verma, N and Raja, Ram and Zaidi , A A (2005) In vitro production of Prunus necrotic ringspot virus-free begonias through chemo- and thermotherapy. Science Horticulture, 103. pp. 239-247.

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Abstract

Abstract Prunus necrotic ringspot virus (PNRSV)-free Begonia spp. plants were raised from petioles of virus-infected plants using in vitro techniques. The petioles were grown onMSmedium supplemented with 0.2 mg/l NAA and 0.2 mg/l BAP (pH 5.8). For rooting, half-strength MS medium without any plant growth regulators was used. On rooting medium, shoots were subjected to chemotherapy (virazole, 2-thiouracil or 6-azauracil) and thermotherapy (38 ◦C for 16 h light period and 22 ◦C for 8 h dark period) separately or in combination. Regenerated plants (treated with chemo- and thermotherapy) were indexed for PNRSV by DAS-ELISA and RT-PCR. An amplified product of 785 bp was obtained by RT-PCR in PNRSV-infected plants. Virazole at a concentration of 20 mg/l was found to be more effective (30 and 20% of PNRSV-free plants as indexed by ELISA and RT-PCR, respectively) in comparison to the other chemicals. Thermotherapy for 25 days gave 35 and 25% PNRSV-free plants as indexed by DAS-ELISA and RT-PCR, respectively. A combination of both treatments gave a good number of PNRSV-free plants (67.5 and 57.5% as indexed by DAS-ELISA and RT-PCR, respectively). At higher concentrations all three chemicals were found to be toxic. Thermotherapy for more than 25 days caused browning of leaves and shoots died.

Item Type:	Article
Uncontrolled Keywords:	Keywords: Begonia; Prunus necrotic ringspot virus; In vitro chemotherapy and thermotherapy
Subjects:	Plant sciences Plant viruses
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	12 Jan 2012 05:40
Last Modified:	07 Feb 2012 11:50
URI:	http://ihbt.csircentral.net/id/eprint/905

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