Mehra, A and Hallan, Vipin and Lal, Brij and Zaidi, A A (2006) Occurrence of Chilli veinal mottle virus in Himalayan butterfly bush ( Buddleja crispa ). Plant Pathology, 55 (2). p. 284.

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Abstract

Buddleja crispa , commonly known as Himalayan butterfly bush, grows in open rocky places. It is a deciduous or semi-evergreen, ornamental, perennial shrub with fuzzy white leaves on long, arching stems, bearing tiny pink, violet or purple flowers. The flowers are often fragrant, attractive and irresistible to butterflies, bees and other nectar-feeding birds and insects. Buddleja davidii , an allied species of B. crispa , has been reported to be a host of Tomato ringspot virus (Hughes & Scott, 2003), Alfalfa mosaic virus (Walter et al ., 1985) and Cucumber mosaic virus (Perkins, 1991). In the course of a study to assess virus incidence in natural plant resources of high-altitude areas in the western Himalayan region of India, some leaves of B. crispa with mild chlorosis and mosaic symptoms were collected from the Kinnaur area (altitude 2500 m asl) of Himachal Pradesh during 2003. The infected leaves were tested by ELISA for the presence of Tomato ringspot virus (antibody kit supplied by DSMZ, Braunschweig, Germany), Cucumber mosaic virus and potyviruses (antibodies from Agdia, Elkhart, USA). A positive result was obtained with the potyvirus group-specific antibodies. To confirm the identity of the virus detected, leaf samples were tested using a universal potyvirus primer pair (P9502 and CPUP), which amplify part of the coat-protein gene and 3 ′ - UTR of the viral genome (Van der Vlugt et al ., 1999). An amplification product of 800 bp was obtained after RT–PCR. The DNA product was cloned, sequenced and submitted to the EMBL Database (accession number AJ889836). The sequence showed 95% homology to Chilli vein-banding mottle virus, a strain of Chilli veinal mottle virus reported from Japan (GenBank accession number AB012221). This is a new report of this potyvirus occurring on B. crispa growing in nature, therefore this plant should be taken into account as a potential reservoir of Chilli veinal mottle virus infecting chilli crops.

Item Type:	Article
Subjects:	Plant sciences Plant viruses
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	02 Jan 2012 07:57
Last Modified:	02 Jan 2012 07:57
URI:	http://ihbt.csircentral.net/id/eprint/741

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