Vyas, D and Kumar, Sanjay (2005) Purification and partial characterization of a low temperature responsive Mn-SOD from tea (Camellia sinensis (L.) O. Kuntze). Biochemical and Biophysical Research Commuications, 329. pp. 831-838.
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Abstract
The manganese containing superoxide dismutase (Mn-SOD) was purified from a tea clone, TEENALI, which showed the lowest period of winter dormancy. Protein was purified using leaves of tea by ammonium sulfate precipitation, followed by column chromatography using DEAE–cellulose, and silica-based size exclusion chromatography on HPLC system. Upto 51-fold purification and a specific activity of 56.66 U/mg of protein was achieved, which yielded a single band upon denaturing PAGE. The enzyme had a native molecular weight of about 169 kDa, whereas a monomer with molecular weight of 43 kDa was found on SDS–PAGE suggesting it to be homotetramer. The purified enzyme had pH optima of 8.0. It exhibited a wide temperature range for its activity with optima at 0 degree C suggesting its role in low temperature tolerance. The manuscript presents purification and characterization of high molecular weight Mn-SOD from tea and discusses its implication in tolerance of low temperature stress.
Item Type: | Article |
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Uncontrolled Keywords: | Camellia sinensis (L.) O. Kuntze; Low temperature; Mn-SOD; Polyclonal antibodies; Purification; TEENALI; Winter dormancy |
Subjects: | Nutraceuticals |
Divisions: | UNSPECIFIED |
Depositing User: | Dr. Aparna Maitra Pati |
Date Deposited: | 30 Dec 2011 05:24 |
Last Modified: | 06 Feb 2012 06:07 |
URI: | http://ihbt.csircentral.net/id/eprint/670 |
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