Rana, T and Chandel, V and Hallan, Vipin and Zaidi, A A (2011) Expression of recombinant Apple chlorotic leaf spot virus coat protein in heterologous system: production and use in immunodiagnosis. Journal of Plant Biochemistry and Biotechnology, 20. pp. 138-141.

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Abstract

Expression condition for maximum recovery of recombinant Apple chlorotic leaf spot virus (ACLSV) coat protein was standardized. The in vitro expressed fusion protein with 6xHis tag (~43 Kd) was purified from inclusion bodies and used as an antigen for raising polyclonal antiserum in rabbit. This antiserum consistently detected ACLSV in pome and stone fruits as well as herbaceous host plants by direct double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) and direct tissue blot immunoassay (DTBIA). The conditions for immuno-capture RT-PCR (IC-RT-PCR) were also standardized.

Item Type:	Article
Uncontrolled Keywords:	Apple chlorotic leaf spot virus . DTBIA . Fusion protein . IC-RT-PCR in vitro expression . Polyclonal antibodies
Subjects:	Plant sciences Plant viruses
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	29 Dec 2011 16:44
Last Modified:	29 Dec 2011 16:44
URI:	http://ihbt.csircentral.net/id/eprint/615

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