Sandal, I and Bhattacharya, Amita and Kumar, Amit and Sharma, Madhu and Shanker, Adarsh and Ahuja, Paramvir Singh (2005) Gradual depletion of 2,4-D in the culture medium for indirect shoot regeneration from leaf explants of Camellia sinensis (L.) O. Kuntze. Plant Growth Regulation, 47 (2-3). pp. 121-127.

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Abstract

Adventitious shoot regeneration via callus phase from in vitro leaf explants is reported for the first time in tea. Callus was obtained on Murashige and Skoog medium supplemented with varied concentrations of 2,4- dichlorophenoxyacetic acid (2,4-D) (2.5, 5.0, 7.5 and 10.0 mg/l). Rhizogenesis was observed at all concentrations of 2,4-D. Adventitious shoot buds developed indirectly on leaf explants after prolonged culture for 16 weeks on medium supplemented with 10.0 mg/l 2,4-D. GC analysis of the medium and the tissues at different stages of development showed that specific levels of 2,4-D in the tissue were responsible for morphogenesis. Shoot buds developed on rhizogenic calli, only when 2,4-D declined to undetectable or negligible concentrations in the tissue probably due to detoxification and metabolism. Alternatively, shoot buds could also be evoked when rhizogenic calli were transferred to medium supplemented with low concentration of 2,4-D (1.5 mg/l). The adventitious nature of the shoots was confirmed through histological studies.

Item Type:	Article
Uncontrolled Keywords:	Callus, Camellia sinensis, 2,4-D, Leaf explant, Shoot regeneration
Subjects:	Plant sciences Plant Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	21 Nov 2011 04:21
Last Modified:	07 Feb 2012 10:32
URI:	http://ihbt.csircentral.net/id/eprint/48

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