Walia, Y and Kumar, Y and Rana, T and Thakur, P D and Sharma, U and Raja, Ram and Hallan, Vipin and Zaidi, A A (2009) Molecular characterization and variability analysis of Apple scar skin viroid in India. Journal of General Plant Pathology, 75. pp. 307-311.

PDF Restricted to Registered users only Download (487Kb)

Abstract

Apple scar skin viroid (ASSVd) infection is a major limitation to apple fruit quality and causes huge economic losses. In surveys of apple orchards in the northern Indian state of Himachal Pradesh, fruits with dappling symptoms were noticed. ASSVd was detected from these fruits and molecularly characterized. Ten clones from three isolates were sequenced, of which seven were new sequence variants of ASSVd. The clones had significant sequence variability (94–100%) with each other. Variability was more common in the pathogenic domain of the viroid genome. Four of the clones were 330 nucleotides (nt) long, and the other six had an additional nucleotide. Phylogenetic analysis showed close affinity of the present isolates with some Chinese and Korean isolates. The study reports seven new variants of ASSVd and also provides the first molecular evidence of viroid infection (ASSVd) in apple in India.

Item Type:	Article
Uncontrolled Keywords:	Apple scar skin viroid. Cloning. DNA sequencing. Phylogenetic analysis
Subjects:	Plant sciences Plant viruses
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	02 Jan 2012 09:54
Last Modified:	02 Jan 2012 09:54
URI:	http://ihbt.csircentral.net/id/eprint/349

Actions (login required)

View Item