Sharma, V and Bhardwaj, P and Sharma, R K and Sood, A and Ahuja, Paramvir Singh (2009) Identification and cross-species amplification of EST derived SSR markers in different bamboo species. Conservation Genetics, 10 (3). pp. 721-724. ISSN 1566-0621

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Abstract

The availability of expressed sequence data derived from gene discovery programs became an alternative source of mining simple sequence repeat (SSR) and developing inexpensive genetic markers for the crop improvements. In present study, 10 express sequence tags (EST)-SSR markers were identified from Bambusa oldhamii public sequence data base. Transferability to 25 species of Bambusoideae ranged from 30% to 100%. The number of alleles detected per locus ranged from 2 to 10. All the newly identified SSR markers were found to be moderately to highly polymorphic with an average Polymorphic Information Content (PIC) value of 0.54. As these loci represents transcribed region and recorded high level of cross transferability and reliable amplification across the species, demonstrating the utility of these markers for functional and genetic analyses of bamboo species.

Item Type:	Article
Uncontrolled Keywords:	Bambusa oldhamii; Bamboo; Cross-amplification; EST-SSR
Subjects:	Nutraceuticals
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	28 Dec 2011 11:36
Last Modified:	28 Dec 2011 11:36
URI:	http://ihbt.csircentral.net/id/eprint/344

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