Hallan, Vipin and Kulshrestha, S and Raikhy, G and Raja, Ram and Zaidi, A A (2006) Occurrenceo f Iris mild mosaicp otyvirus in cultivatedi ris in India. Journal of Biotechnology, 5. pp. 94-98.
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Abstract
Iris (Iris x hollandica Hort. cv. Bluemagic)p lants showing mosaic symptomsw ere testedf or the presenceo f Iris mild mosaic polyvirus (IMMV) by host range, enzyme linked immunosorbent assay (ELISA) using antibodies specific for IMMV), reverset ranscriptionp olymerasec hain reaction (RT-PCR),i mmunocapturere verset ranscriptionp olymerasec hain reaction (IC-RT -PCR) using potyvirus group specific primers and antibodies specific to IMMV, immune electron microscopy and cytopathology. Presence of IMMV was detected by ELISA, confirmed by RT-PCR, IC-RT-PCR, IEM, cytopathologya nd sequencingo f the cloned PCR product. From the sequenceo f the virus, specific primers were designed and specific detection of IMMV from iris was standardized
Item Type: | Article |
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Uncontrolled Keywords: | cytopathology, LISA, IC-RT-PCR, IEM, Iris mild mosaic polyvirus(IMMV) |
Subjects: | Plant Biotechnology Plant viruses |
Divisions: | UNSPECIFIED |
Depositing User: | Dr. Aparna Maitra Pati |
Date Deposited: | 29 Dec 2011 10:34 |
Last Modified: | 29 Dec 2011 10:34 |
URI: | http://ihbt.csircentral.net/id/eprint/242 |
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