Sharma, A and Mahinaghara, B K and Singh, A K and Kulshrestha, S and Raikhy, G and Singh, L and Hallan, Vipin and Verma, N and Raja, Ram and Zaidi, A A (2005) Identification, detection and frequency of lily viruses in Northern India. Scientia Horticulturae, 106 (2). pp. 213-227.

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Abstract

In a survey of lily growing fields in various parts of Himachal Pradesh, India, three viruses, Cucumber mosaic (CMV), Lily symptomless (LSV) and Lily mottle virus (LMoV) lily strain, were found quite prevalent in Asiatic (12 cultivars) and Oriental hybrids (4 cultivars) of lily, Lilium longiflorum and L. tigrinum. Apart from these viruses, Strawberry latent ringspot virus (SLRSV) was also found to infect Oriental hybrid lily. The disease incidence on the basis of symptoms observed ranged between minimum (40.7%) in cv. Alaska and maximum (83.7%) in cv. America, both Asiatic hybrid lily types. These viruses were indexed by testing outer and inner scales of bulbs and the leaves at early and flowering stages using ELISAand reverse transcription polymerase chain reaction (RT-PCR). Mechanical and insect transmission, purification, electron microscopy and sequencing of the PCR fragments were carried out for these viruses. Cloning and sequence analysis confirmed the viruses as CMV-subgroup II, LSVandLMoV. There were 96–98%nucleotide and 93–97% amino acid homology with CMV subgroup II sequences, 97–99% nucleotide and 91–92% amino acid homology with LSV sequences and 92–99% nucleotide and 85–98% amino acid homology with LMoV sequences

Item Type:	Article
Uncontrolled Keywords:	Lily; CMV; LSV; LMoV; SLRSV; ELISA; EM; RT-PCR; Sequencing
Subjects:	Plant sciences Plant viruses
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	30 Dec 2011 03:41
Last Modified:	13 Feb 2012 05:50
URI:	http://ihbt.csircentral.net/id/eprint/220

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