Chandel, V and Rana, T and Thakur, P D and Hallan, Vipin and Handa, A and Zaidi, A A (2008) Incidence of Prunus necrotic ring spot virus on Malus domestica in India. Journal of Phytopathology, 156 (6). pp. 382-384.

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Abstract

In surveys of apple (Malus domestica) orchards in various parts of Himachal Pradesh, samples from trees showing necrotic symptoms on the leaves were collected and tested for detection of Prunus necrotic ringspot virus (PNRSV) initially by ELISA followed by RT-PCR using coat protein gene primers. Positive results were obtained in samples from Kullu and Kalpa regions. The virus gene sequences showed 88–97% similarity to corresponding sequences of other PNRSV isolates deposited in the GenBank database using ncbi.nih.nlm.gov. Although the similarity was high, there were some distinct differences with the Spanish isolate. This is the first report of PNRSV in apple from India.

Item Type:	Article
Uncontrolled Keywords:	PNRSV, apple, RT-PCR, ELISA, India
Subjects:	Plant sciences
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	02 Jan 2012 10:05
Last Modified:	02 Jan 2012 10:05
URI:	http://ihbt.csircentral.net/id/eprint/171

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