Kalia, K and Sharma, K and Singh, H P and Singh , Bikram (2008) Effects of Extraction Methods on Phenolic Contents and Antioxidant Activity in Aerial Parts of Potentilla atrosanguinea Lodd. and Quantification of Its Phenolic Constituents by RP-HPLC†. Journal of Agricultural and Food Chemistry, 56 (21). pp. 10129-10134.

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Abstract

The effects of different solvent systems (methanol, ethanol, acetone, and their 50% aqueous concentrations) and extraction procedures (microwave, ultrasound, Soxhlet and maceration) on the antioxidant activity of aerial parts of Potentilla atrosanguinea were investigated by three different bioassays: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2′- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays and ferric reducing antioxidant potential (FRAP). The 50% aqueous ethanol extracts exhibited strong antioxidant activity measured in terms of Trolox equivalent antioxidant capacity (TEAC) [(54.34 to 122.96, 29.82 to 101.22 and 13.64 to 41.43) mg of Trolox/g] with ABTS•+, DPPH• and FRAP assays, respectively. In general, TEAC of Soxhlet extracts was found to be 1.8 and 3 times higher than ultrasound and maceration but slightly (1.2 times) higher than microwave. A positive correlation (r2 ) 0.931 to 0.982) was observed between total polyphenol (TPC) and total flavonoid (TFC) contents which ranged between 26.7 to 30.7 mg/g gallic acid equivalent and 16.8 to 20.8 mg/g quercetin equivalent respectively, with antioxidant activity. In addition, some of its bioactive phenolic constituents which contribute largely toward antioxidant potential such as chlorogenic acid, catechin, caffeic acid, p-coumaric acid and quercetin were also quantified in different extracts by RP-HPLC.

Item Type:	Article
Uncontrolled Keywords:	Potentilla atrosanguinea (Lodd.); polyphenols; MAE; Soxhlet; antioxidant activity; HPLC
Subjects:	Nutraceuticals Natural Product Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	29 Dec 2011 07:21
Last Modified:	29 Dec 2011 07:21
URI:	http://ihbt.csircentral.net/id/eprint/125

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