Saini, Rikki and Jaitaka, Vikas and Guleriaa, Shailja and Kaul, V K and Kiran Babu, GD and Singh , Bikram and Lal, Brij and Singh, R D (2012) Comparison of headspace analysis of volatile constituents with GC–MS analysis of hydrodistilled and supercritical fluid extracted oil of Capillipedium parviflorum. The Journal of Essential Oil Research, 24 (3). pp. 315-320.

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Abstract

Volatile constituents of the aerial parts of Capillipedium parviflorum were studied by two different extraction procedures, hydrodistillation (HD), supercritical CO2 extraction (Sc-CO2) and compared with headspace analysis (HS). Sc- CO2 extraction was carried out using nine differential optimization extractions (DOX) at different temperatures (°C) and pressures (MPa), i.e. 35/9, 60/9, 85/9, 35/17, 60/17, 85/17, 35/25, 60/25 and 85/25, to assess the influence on the yield and composition of volatile constituents. In DOX experiments, oil yields varied between 0.01–0.18% and maximum yield (0.18%) was obtained using temperatures and pressures 35/17 and 85/25. The oil is characterized by distinctive presence of very high content of non-terpenes (90.7%) identified in HD oil, 84.0% in Sc-CO2 oil and 92.5% in HS analysis. Monoterpenes were represented in low concentrations in HD oil and were totally absent in Sc- CO2 as well as in HS analysis. Very little variability was observed in the oxygenated monoterpenes in both Sc-CO2 and HD oil representing a low percentage of constituents. Sesquiterpene hydrocarbons were represented between 1.5% and 3.1% in Sc-CO2 extraction, which was higher in comparison with HD and HS techniques. Oxygenated sesquiterpenes were represented in higher percentage in Sc-CO2 than in HD oil and HS analysis. Variability among the major constituents of 4-nonanone, 4-nonanol, 4-undecanone and 4-undecanol was observed in Sc-CO2 extraction carried out under varied temperatures and pressures. Their percentage also varied in HD and HS analysis. 4-Nonanone (2.5%, 60/9 in Sc-CO2; 6.4% in HD and 4.3% in HS), 4-nonanol (21.5% in Sc-CO2, 31.7% in HD and 29.5% in HS), 4-undecanone (33.3%, 85/9 in Sc-CO2; 35.3% in HD and 44.5% in HS) and 4-undecanol (16.5%, 84/17, 60/25 in Sc-CO2; 14.6% in HD and 10.3% in HS) dominated the volatile constituents.

Item Type:	Article
Uncontrolled Keywords:	Capillipedium parviflorum; Poaceae; Sc-CO2; hydrodistillation; headspace analysis; comparative evaluation
Subjects:	Natural Product Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	03 Jan 2013 10:49
Last Modified:	03 Jan 2013 10:49
URI:	http://ihbt.csircentral.net/id/eprint/1241

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