kumar, Vinay and Gill, T and Grover, Sunita and Ahuja, Paramvir Singh and Yadav , S K (2012) Influence of Human Lactoferrin Expression on Iron Homeostasis, Flavonoids, and Antioxidants in Transgenic Tobacco. Mol Biotechnol.

PDF Restricted to Registered users only Download (741Kb)

Abstract

This study was aimed at to check the influence of human lactoferrin (hLF) expression on iron homeostasis, flavonoids, and antioxidants in transgenic tobacco. Transgenic tobacco expressing hLF cDNA under the control of a CaMV 35S promoter was produced. The iron content as well as chlorophyll content of transgenic tobacco was lower compared to mock and untransformed wild plants. Interestingly, hLF transgenic tobacco showed higher level of transcript expression for genes related to iron content regulation like iron transporter and metal transporter. While expression of genes related to iron storage such as ferritin 1 and ferritin 2 was downregulated. The transcript expression of genes encoding antioxidant enzymes such as glutathione reductase, glutathione-S-transferase, ascorbate peroxidase, and catalase was downregulated in hLF transgenic tobacco compared to controls. Further, the transcript expression of two important genes encoding dihydroflavonol reductase (DFR) and phenylalanine ammonia lyase regulatory enzymes of flavonoid biosynthesis pathway was analyzed. The expression of DFR was found to be downregulated, while PAL expression was upregulated in hLF transgenic tobacco compared to mock and untransformed wild plant. Total phenolics, flavonoids, and proanthocyanidins contents were found to be higher in hLF transgenic tobacco than the mock and untransformed wild plant

Item Type:	Article
Uncontrolled Keywords:	Antioxidants. Human lactoferrin. Total flavonoids. Iron homeostasis
Subjects:	Plant sciences Plant Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	27 Apr 2012 06:00
Last Modified:	27 Apr 2012 06:00
URI:	http://ihbt.csircentral.net/id/eprint/1083

Actions (login required)

View Item