Kaur, D and Ogra, R K and Bhattacharya, Amita and Sood, A (2012) Changes in sugar levels during slow growth of Dendrocalamus hamiltonii somatic embryos due to liquid paraffin overlay. In Vitro Cell.Dev.Biol, 48. pp. 120-126.

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Abstract

A liquid paraffin overlay (LPO) was used for storage of rapidly multiplying somatic embryos of Dendrocalamus hamiltonii under slow-growth conditions. Slow growth was associated with changes in sugar metabolism. In rapidly growing embryogenic tissues, a sharp decline in starch and non-reducing sugars indicated rapid utilization of starch. In contrast, under slow-growth conditions in somatic embryos stored under LPO, a gradual decline in starch indicated its slower utilization. As a result, growth of somatic embryos under LPO was suppressed and subculturing was not required. Following retrieval from growth under LPO after 30, 90, 180, 270, and 365 d of storage, the somatic embryos showed high recovery and germination (79.78%, 77.49%, 71.22%, 67.13%, and 59.99%, respectively) and were able to proliferate following transfer to Murashige and Skoog’s (Physiol. Plant. 15:473–497, 1962) medium containing 1 mgl−1 BAP and 2% sucrose. The study provides useful information on in vitro storage of embryogenic tissue of D. hamiltonii.

Item Type:	Article
Uncontrolled Keywords:	Dendrocalamus hamiltonii . Liquid paraffin overlay . Slow growth . Soluble sugars and starch
Subjects:	Plant sciences Plant Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	23 Apr 2012 05:38
Last Modified:	23 Apr 2012 05:38
URI:	http://ihbt.csircentral.net/id/eprint/1053

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