Hallan, Vipin and Kulshrestha, S and Raikhy, G and Raja, Ram and Zaidi, A A (2006) Occurrenceo f Iris mild mosaicp otyvirus in cultivatedi ris in India. Journal of Biotechnology, 5. pp. 94-98.

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Iris (Iris x hollandica Hort. cv. Bluemagic)p lants showing mosaic symptomsw ere testedf or the presenceo f Iris mild mosaic polyvirus (IMMV) by host range, enzyme linked immunosorbent assay (ELISA) using antibodies specific for IMMV), reverset ranscriptionp olymerasec hain reaction (RT-PCR),i mmunocapturere verset ranscriptionp olymerasec hain reaction (IC-RT -PCR) using potyvirus group specific primers and antibodies specific to IMMV, immune electron microscopy and cytopathology. Presence of IMMV was detected by ELISA, confirmed by RT-PCR, IC-RT-PCR, IEM, cytopathologya nd sequencingo f the cloned PCR product. From the sequenceo f the virus, specific primers were designed and specific detection of IMMV from iris was standardized

Item Type: Article
Uncontrolled Keywords: cytopathology, LISA, IC-RT-PCR, IEM, Iris mild mosaic polyvirus(IMMV)
Subjects: Plant Biotechnology
Plant viruses
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 29 Dec 2011 10:34
Last Modified: 29 Dec 2011 10:34
URI: http://ihbt.csircentral.net/id/eprint/242

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