Kaur, D and Ogra, R K and Bhattacharya, Amita and Sood, A (2012) Changes in sugar levels during slow growth of Dendrocalamus hamiltonii somatic embryos due to liquid paraffin overlay. In Vitro Cell.Dev.Biol, 48. pp. 120-126.
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Abstract
A liquid paraffin overlay (LPO) was used for storage of rapidly multiplying somatic embryos of Dendrocalamus hamiltonii under slow-growth conditions. Slow growth was associated with changes in sugar metabolism. In rapidly growing embryogenic tissues, a sharp decline in starch and non-reducing sugars indicated rapid utilization of starch. In contrast, under slow-growth conditions in somatic embryos stored under LPO, a gradual decline in starch indicated its slower utilization. As a result, growth of somatic embryos under LPO was suppressed and subculturing was not required. Following retrieval from growth under LPO after 30, 90, 180, 270, and 365 d of storage, the somatic embryos showed high recovery and germination (79.78%, 77.49%, 71.22%, 67.13%, and 59.99%, respectively) and were able to proliferate following transfer to Murashige and Skoog’s (Physiol. Plant. 15:473–497, 1962) medium containing 1 mgl−1 BAP and 2% sucrose. The study provides useful information on in vitro storage of embryogenic tissue of D. hamiltonii.
Item Type: | Article |
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Uncontrolled Keywords: | Dendrocalamus hamiltonii . Liquid paraffin overlay . Slow growth . Soluble sugars and starch |
Subjects: | Plant sciences Plant Biotechnology |
Divisions: | UNSPECIFIED |
Depositing User: | Dr. Aparna Maitra Pati |
Date Deposited: | 23 Apr 2012 05:38 |
Last Modified: | 23 Apr 2012 05:38 |
URI: | http://ihbt.csircentral.net/id/eprint/1053 |
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