Swaroop, A and Gupta , A P and Sinha, Arun K (2005) Simultaneous determination of quercetin, rutin and coumaric acid in flowers of Rhododendron arboreum by HPTLC. Chromatographia, 62 (11-12). pp. 649-652. ISSN 0009-5893

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Abstract

A simple and fast method was developed for simultaneous quantitative determination of three biologically active phenolic compounds i.e. quercetin, rutin and coumaric acid in flowers of Rhododendron orboreum using high-performance thin-layer chromatography (HPTLC). The separation was performed on TLC aluminium plates precoated with silica gel RP-18 F-254S. Good separation was achieved in the mobile phase of methanol-water-formic acid (40:57:3, v/v/v) and densitometric determination of these compounds was carried out at 280 nm in reflectance/absorbance mode. The linear regression data for the calibration plots showed a good linear relationship with r = 0.9971, 0.9953 and 0.9960 quercetin, rutin and coumaric acid, respectively. Accuracy of the method was checked by recovery study conducted at two different levels with the average recovery of 99.90%, 99.02% and 99.16% for quercetin, rutin and cournaric acid, respectively. The present method is being reported first time and may be used for routine quality control of the flowers of R. arboreum.

Item Type:	Article
Uncontrolled Keywords:	Thin-layer chromatography; Rhododendron arboreum Quercetin, rutin and coumaric acid.
Subjects:	Chemical Engineering
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	27 Dec 2011 11:42
Last Modified:	27 Dec 2011 11:42
URI:	http://ihbt.csircentral.net/id/eprint/184

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