Sharma, O P and Kumar, Neeraj and Bhat., Tej. K and Singh , Bikram (2012) An improved method for thin layer chromatographic analysis of saponins. Journal of Food Chemistry, 132. pp. 671-674.

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a b s t r a c t Analysis of saponins by thin layer chromatography (TLC) is reported. The solvent system was n-butanol: water:acetic acid (84:14:7). Detection of saponins on the TLC plates after development and air-drying was done by immersion in a suspension of sheep erythrocytes, followed by washing off the excess blood on the plate surface. Saponins appeared as white spots against a pink background. The protocol provided specific detection of saponins in the saponins enriched extracts from Aesculus indica (Wall. ex Camb.) Hook.f., Lonicera japonica Thunb., Silene inflata Sm., Sapindus mukorossi Gaertn., Chlorophytum borivilianum Santapau & Fernandes, Asparagus adscendens Roxb., Asparagus racemosus Willd., Agave americana L., Camellia sinensis [L.] O. Kuntze. The protocol is convenient, inexpensive, does not require any corrosive chemicals and provides specific detection of saponins

Item Type: Article
Uncontrolled Keywords: Keywords: Thin layer chromatography Saponins Haemolysis
Subjects: Plant sciences
Natural Product Chemistry
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 10 Feb 2012 10:13
Last Modified: 10 Feb 2012 10:13

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