Bhardwaj, P K and Kaur, J and Sobit, R C and Ahuja, Paramvir Singh and Kumar, Sanjay (2011) Lipoxygenase in Caragana jubata responds to low temperature, abscisic acid, methyl jasmonate and salicylic acid. Gene, 483. pp. 49-53.

PDF Restricted to Registered users only Download (2045Kb)

Abstract

Lipoxygenase (LOX) catalyses oxygenation of free polyunsaturated fatty acids into oxylipins, and is a critical enzyme of the jasmonate signaling pathway. LOX has been shown to be associated with biotic and abiotic stress responses in diverse plant species, though limited data is available with respect to low temperature and the associated cues. Using rapid amplification of cDNA ends, a full-length cDNA (CjLOX) encoding lipoxygenase was cloned from apical buds of Caragana jubata, a temperate plant species that grows under extreme cold. The cDNA obtained was 2952 bp long consisting of an open reading frame of 2610 bp encoding 869 amino acids protein. Multiple alignment of the deduced amino acid sequence with those of other plants demonstrated putative LH2/ PLAT domain, lipoxygenase iron binding catalytic domain and lipoxygenase_2 signature sequences. CjLOX exhibited up- and down-regulation of gene expression pattern in response to low temperature (LT), abscisic acid (ABA), methyl jasmonate (MJ) and salicylic acid (SA). Among all the treatments, a strong up-regulation was observed in response to MJ. Data suggests an important role of jasmonate signaling pathway in response to LT in C. jubata.

Item Type:	Article
Uncontrolled Keywords:	Keywords: ABA Differential display Jasmonate Lipoxygenase Low temperature Salicylate
Subjects:	Plant sciences Natural Product Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	09 Feb 2012 09:17
Last Modified:	09 Feb 2012 09:17
URI:	http://ihbt.csircentral.net/id/eprint/956

Actions (login required)

View Item