Guleria, S and Jaitak, V and Saini, R and Kaul , V K and Lal, Brij and G D, Kiran Babu and Singh , Bikram and Singh, R D (2011) Comparative studies of volatile oil composition of Rhododendron anthopogon by hydrodistillation, supercritical carbon dioxide extraction and head space analysis. Natural Product Research, 25 (13). pp. 1271-1277.

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Abstract

Volatile oil composition of the leaves of Rhododendron anthopogon (Ericaceae) growing wild in alpine Western Himalaya was studied using different extraction techniques including SC-CO2 extraction and hydrodistillation (HD). Results from different extraction methodologies were compared with headspace analysis (HS) and evaluated for the effectiveness of techniques in characterisation of various terpene categories and to assess their influence on the yield and composition of volatiles. Variability in constituents and in quantitative yields was observed. The results varied with different extraction methods. A total of 27 constituents in SC-CO2 extraction, 31 in HD and 17 in HS analysis were identified. Constituents in SC-CO2 and HD oils were identified by gas chromatography mass spectrometry analysis. SC-CO2 extraction was carried out at 40 degree C and 140 bar pressure and the oil represented by major constituents as �-caryophyllene (5.96%), �-humulene (4.06%) and p-menthadiene-2,9- diol (7.28%); in HD, oil limonene (11.26%), �-caryophyllene (11.62%), �-humulene (7.22%), and E-nerolidol (5.83%) dominated the oil and in HS analysis, limonene (24.14%), �-terpinene (40.73%), �-terpinene (4.92%), �-phellandrene (3.44%) and �-ocimene (7.15%) were present as major constituents

Item Type:	Article
Uncontrolled Keywords:	Rhododendron anthopogon; Ericaceae; volatile oil; SC-CO2; hydrodistillation; GC–MS; headspace analysis; comparison
Subjects:	Plant sciences Natural Product Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	08 Feb 2012 05:54
Last Modified:	08 Feb 2012 05:54
URI:	http://ihbt.csircentral.net/id/eprint/943

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