Kumar , Anil and Sood, A and Palni T, Uma and Gupta , A K and Palni, LMS (2001) Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron. Journal of Horticultural Science & Biotechnology, 76 (1). pp. 30-34.

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Summery An efficient protocol for micropropagation of Rosa damascena Mill.has been established using single node segments from mature bushes. The effect of thidiazuron has been compared with that of N6-benzyladenine(BA)on in vitro shoot proliferation.The cultures initiated on medium supplemented with thidiazuron(TDZ)and/or cultured continuously on TDZ containing medium for 32-48 weeks exhibited considerably more shoot proliferation and growth during subsequent culture on a medium containing BA. Shoots induced on TDZ containing medium and then sub-cultured(8-12 times)on medium containing BA,attained the capacity to grow and proliferate on a medium free from plant growth regulators(PGR). Microshoots from TDZ induced cultures could be rooted easily on indole-3-butyric acid(IBA) supplemented medium. A short treatment with IBA(100uM;12h)was found to be very effective for root induction. The rooted plants were successfully transferred to pots after 15 d of hardening in a mist chamber with about 70% survival.

Item Type: Article
Uncontrolled Keywords: Rosa damascena;
Subjects: Plant sciences
Plant Biotechnology
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 10 Jan 2012 04:23
Last Modified: 10 Jan 2012 04:23
URI: http://ihbt.csircentral.net/id/eprint/895

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