Kumar , Anil and Sood, A and Palni T, Uma and Gupta , A K and Palni, LMS (2001) Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron. Journal of Horticultural Science & Biotechnology, 76 (1). pp. 30-34.

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Abstract

Summery An efficient protocol for micropropagation of Rosa damascena Mill.has been established using single node segments from mature bushes. The effect of thidiazuron has been compared with that of N6-benzyladenine(BA)on in vitro shoot proliferation.The cultures initiated on medium supplemented with thidiazuron(TDZ)and/or cultured continuously on TDZ containing medium for 32-48 weeks exhibited considerably more shoot proliferation and growth during subsequent culture on a medium containing BA. Shoots induced on TDZ containing medium and then sub-cultured(8-12 times)on medium containing BA,attained the capacity to grow and proliferate on a medium free from plant growth regulators(PGR). Microshoots from TDZ induced cultures could be rooted easily on indole-3-butyric acid(IBA) supplemented medium. A short treatment with IBA(100uM;12h)was found to be very effective for root induction. The rooted plants were successfully transferred to pots after 15 d of hardening in a mist chamber with about 70% survival.

Item Type:	Article
Uncontrolled Keywords:	Rosa damascena;
Subjects:	Plant sciences Plant Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	10 Jan 2012 04:23
Last Modified:	10 Jan 2012 04:23
URI:	http://ihbt.csircentral.net/id/eprint/895

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