Kaur, P and Kaur, S and Kumar, Neeraj and Singh , Bikram and Kumar, Sanjay (2009) Evaluation of antigenotoxic activity of isoliquiritin apioside from Glycyrrhiza glabra L. Toxicology in vitro, 23. pp. 680-686.

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Abstract

a b s t r a c t Prevention of manifestation of events characteristic of carcinogenesis is being emphasized a rational strategy to combat cancer. Reactive oxygen species (ROS) play an important role in tumor initiation through oxidative damage of DNA. In search for lead molecules in cancer chemoprevention from natural products, a fraction ‘Rlicca’ isolated from Glycyrrhiza glabra was studied for modulatory effect against hydrogen peroxide and 4-nitroquinoline-N-oxide induced genotoxicity in Escherichia coli PQ37 using SOS chromotest and in human peripheral blood lymphocytes using the Comet assay. The fraction ‘Rlicca’ at a concentration of 191 lM decreased the SOS inducing potency (SOSIP) of hydrogen peroxide (1.0 mM) and NQO (20 lg/ml) by 83.72% and 68.77%, respectively. In the human blood lymphocytes, ‘Rlicca’ reduced the tail moment induced by hydrogen peroxide (25 lM) and NQO (5 lg/ml) by 88.04% and 76.64%, respectively, using the Comet assay. The spectroscopic data of ‘Rlicca’ fraction revealed it to be isoliquiritin apioside, a chalcone oligoglycoside. This is the first report of isoliquiritin apioside with marked potential to combat oxidative stress-induced genotoxicity.

Item Type:	Article
Uncontrolled Keywords:	Keywords: Glycyrrhiza glabra Isoliquiritin apioside SOS chromotest Comet assay Escherichia coli PQ37 Human lymphocyte
Subjects:	Natural Product Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	06 Jan 2012 11:28
Last Modified:	06 Feb 2012 06:18
URI:	http://ihbt.csircentral.net/id/eprint/892

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