Malik, S and Sharma, N and Sharma , U K and Singh , NP and Bhushan, Shashi and Sharma, Madhu and Sinha, Arun K and Ahuja, Paramvir Singh (2010) Qualitative and quantitative analysis of anthraquinone derivatives in rhizomes of tissue culture-raised Rheum emodi Wall. plants. Journal of Plant Physiology, 167. pp. 749-756.

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This paper presents quantification of five anthraquinone derivatives (emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion) in rhizomes of hardened micro-propagated Rheum emodi plants using high-performance liquid chromatography (HPLC). Aseptic shoot cultures were raised using rhizome buds. Shoot multiplication occurred in both agar gelled and liquid Murashige and Skoog (MS) medium supplemented with 10.0 μM 6-benzylaminopurine (BAP) and 5.0 μM indole-3-butyric acid (IBA). Rooted plantlets obtained on plant growth regulator (PGR)-free medium were transferred to soil with 92% survival. HPLC analysis revealed the presence of five anthraquinone derivatives: emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion in rhizomes of tissue culture-raised plants. Only emodin glycoside (1) and chrysophanol glycoside (2) were present in 6-month-old hardened tissue cultured plants. In addition, the other three derivatives (emodin (3), chrysophanol (4) and physcion (5)) were also detected after 9 months.

Item Type: Article
Uncontrolled Keywords: Anthraquinone; Plant tissue culture; Polygonaceae; Rheum emodi; Secondary metabolite
Subjects: Plant Biotechnology
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 02 Jan 2012 10:28
Last Modified: 10 Feb 2012 07:49

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