Mondal, T K and Bhattacharya, Amita and Ahuja, Paramvir Singh (2001) Induction of synchronous secondary somatic embryogenesis in Camellia sinensis (L.) O. Kuntze. J.Pl.Physiol., 158. pp. 945-51.

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Nutritional effect of nitrate salts of potassium and ammonium, together with different concentrations of sulphate salts of aluminium, potassium, magnesium, and ammonium on secondary somatic embryogenesis, wereinvestigated. Nitrate salts of potassium (9.39 mmol/L) and ammonium (10.31 mmol/L) with only 1.5 mmol/L potassium sulphate produced maximum number of synchronous secondary embryos (i.e. 20-25 secondary embryos per primary embryo in 91.6 percnt; responsive explants). Of the different factorial combinations of glutamine, BAP, and IBA tested, maximum number of synchronous secondary embryos developed on a medium supplemented with 8.88 μmol/L BAP, 0.98 μmol/L IBA and 10 mmol/L glutamine. Synchronous and normal development of secondary embryos could thus be obtained when optimal concentrations of PGRs, glutamine, nitrates, and salts of potassium sulphate were combined together. Germination of the embryos (up to 52 percnt;) was acheived only when sulphate salts of potassium were removed from the medium.

Item Type: Article
Uncontrolled Keywords: nitrate salts; sulphate salts; synchronous secondary embryos; Camellia sinensis
Subjects: Plant Biotechnology
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 30 Dec 2011 03:13
Last Modified: 30 Dec 2011 03:13

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