Godbole, S and Sood, A and Thakur, R and Sharma, Mohit and Ahuja, Paramvir Singh (2002) Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo, Dendrocalamus hamiltonii Nees et Arn. Ex Munro. Current Science, 83. pp. 885-9.

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Abstract

New sprouts from the nodal segments of the field-selected, mature, elite bamboos were used for the induction of embryogenic callus. Murashige and Skoog medium with BA and 2,4-D (1.0 mg/l each) was essential for culture establishment and callusing. Subsequently, elimination of 2,4-D and a corresponding increase in BA concentration induced embryogenesis. Upon transfer to sucrose-enriched medium without PGRs, these embryos matured and germinated into plantlets within 21 days, with a conversion rate of about 80%. The protocol thus established showed a low frequency of albinos when embryos were matured on a high sucrose (8%) containing medium under diffuse light conditions (5 mumol m(-2) S-1). Rhizome formation took place upon prolonged culturing in the same medium and served as excellent propagules for multiplication. The plantlets were hardened and up to 78% survival rate was achieved in soil under greenhouse conditions.

Item Type:	Article
Uncontrolled Keywords:	TISSUE-CULTURE; STRICTUS NEES; VITRO; REGENERATION; PROPAGATION
Subjects:	Plant Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	30 Dec 2011 03:08
Last Modified:	10 Feb 2012 07:39
URI:	http://ihbt.csircentral.net/id/eprint/631

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