Sood, Priyanka and Bhattacharya, Amita and Sood, A (2011) Problems and possibilities of monocot transformation. Biologia Plantarum, 55 (1). pp. 1-15.

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Abstract

Biotechnological improvement of monocots is often hampered by the lack of efficient regeneration systems, requisite wound responses and low cell competence. Despite these limitations, the biolistic and Agrobacterium methods have been successfully used to produce several transgenic monocots by adjusting the parameters that govern efficient delivery and integration of transgene(s) into plant genome. It is now possible to transform even difficult monocots using tailor-made gene constructs and promoters, suitable A. tumefaciens strains and a proper understanding of the entire process. This success has been reviewed in the present article and a special emphasis was laid on the measures that were taken in overcoming the difficulties that arise due to the differential responses of monocots and dicots. This information is necessary for biotechnological improvement of still newer monocotyledonous plants that have been hitherto difficult to transform.

Item Type:	Article
Uncontrolled Keywords:	Agrobacterium, microprojectile bombardment, tailor-made gene constructs.
Subjects:	Plant Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	30 Dec 2011 03:07
Last Modified:	09 Apr 2013 07:59
URI:	http://ihbt.csircentral.net/id/eprint/624

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