Sharma , N and Sharma, U K and Malik, S and Bhushan, Shashi and Kumar, Vinod and Verma , S C and Sharma, N and Sharma, Madhu and Sinha, Arun K (2008) Isolation and purification of acetylshikonin and b-acetoxyisovalerylshikonin from cell suspension cultures of Arnebia euchroma (Royle) Johnston using rapid preparative HPLC. Journal of Separation Science, 31. pp. 629-635.

[img] PDF
Restricted to Registered users only

Download (952Kb)


Shikonin and its derivatives are important red colored naphthoquinone pigments found in a large number of Arnebia species, including A. euchroma, that are responsible for the various pharmacological activities exhibited by the plant. The precise separation of each naphthoquinone is essential for total quality evaluation and bioactivity analysis of herbal formulations of A. euchroma. Furthermore, the overexploitation of this useful plant has resulted in species becoming endangered. With this in mind, a simple and rapid preparative scale HPLC method with single compound recovery for the isolation and purification of two shikonin derivatives (i. e. acetylshikonin, b-acetoxyisovalerylshikonin) from cell suspension cultures of A. euchroma is presented. The compounds were separated on a C18 column within 10 min using acetonitrile/ methanol (95:5) as mobile phase in isocratic mode. The isolated compounds were found to be more than 98% pure. The LOD for acetylshikonin and bacetoxyisovalerylshikonin was estimated at 0.063 and 0.146 lg/mL, respectively, while the LOQ was found to be 0.209 and 0.487 lg/mL, respectively. The recoveries accomplished for both the shikonin derivatives were in the range of 94.7–96.8%. The repeatability, expressed as %RSD, of acetylshikonin and b-acetoxyisovalerylshikonin was found to be 1.74 and 1.27, respectively.

Item Type: Article
Uncontrolled Keywords: Acetylshikonin / b-Acetoxyisovalerylshikonin / Preparative HPLC / Arnebia euchroma / Cell suspension cultures /
Subjects: Natural Product Chemistry
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 29 Dec 2011 15:54
Last Modified: 02 Jan 2013 12:26

Actions (login required)

View Item View Item