Bhandari, P and Singh , Bikram and Gupta , A P and Kaul , V K (2006) HPTLC Determination of Swertiamarin and Amarogentin in Swertia Species from the Western Himalayas. JPC-Journal of Planar Chromatography-Modern TLC , 19 (109). pp. 212-215. ISSN 0933-4173

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Abstract

A high-performance thin-layer chromatographic (HPTLC) method has been established for simple and rapid quantification of two secoiridoids, swertiamarin and amarogentin, in two Swertia species. HPTLC of secoiridoids was performed on precoated silica 60 F254 plates with EtOAc–MeOH–H2O, 77 + 8 + 8 (v/v), as mobile phase. Densitometric determination of the secoiridoids was performed at λ = 235 nm in reflectance/absorbance mode. The method was validated for precision, repeatability, and accuracy. Average recovery of amarogentin and swertiamarin was 94.5 and 96.5%, respectively, showing that the reproducibility of the method was excellent. The method is simple, precise, specific, sensitive, and accurate and can be used for routine quality control of raw materials and herbal formulations.

Item Type:	Article
Uncontrolled Keywords:	Swertia chirata; Swertia cordata; Secoiridoids; Amarogentin Swertiamarin; HPTLC
Subjects:	Natural Product Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	27 Dec 2011 08:30
Last Modified:	27 Dec 2011 08:30
URI:	http://ihbt.csircentral.net/id/eprint/51

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