Pati, P K and Sharma, Madhu and Sood, A and Ahuja, Paramvir Singh (2004) DIRECT SHOOT REGENERATION FROM LEAF EXPLANTS OF ROSA DAMASCENA MILL. in Vitro Plant Cellular and Developmental Biology, 40 (2). pp. 192-195.

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A protocol for in vitro propagation using direct induction of shoot buds from leaf explants of in vitro-raised shoots of Rosa damascena var. Jwala is reported. The present study is the first report on direct shoot regeneration in scented roses. Elite plants raised from nodal explants and maintained for over 2 yr in vitro on a static liquid shoot multiplication Murashige and Skoog (MS) medium supplemented with 5.0 mM benzyladenine (BA) and 3% sucrose were used. Petioles from fully developed young leaves, obtained after 4 wk of pruning of old shoots, were found to be ideal for regeneration of shoots. Initially the explants were cultured in an induction medium [half-strength MS þ 3%sucrose þ 6.8mM thidiazuron þ 0.27mM a-naphthaleneacetic acid (NAA) þ 17.7mM AgNO3] and subsequently transferred to the regeneration medium (MS þ 2.25mM BA þ 0.054mM NAA) after 7, 14, 21, 28, and 35 d. The highest shoot regeneration response (69%) was recorded when shoots were kept in the induction medium for 21 d and later transferred to regeneration medium. Histological studies revealed direct formation of shoot buds without the intervening callus phase. In vitro rooting of micro-shoots was accomplished within 2 wk on half-strength MS liquid medium supplemented with 10.0 mM IBA and 3% sucrose for 1 wk in the dark and later transferred to hormone-free medium and kept in the light. Plantlets, remaining in the latter medium for 5–6 wk when transferred to soil, showed 90% survival.

Item Type: Article
Uncontrolled Keywords: direct regeneration; histology; rooting; leaf; Rosa damascena.
Subjects: Plant Biotechnology
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 29 Dec 2011 11:41
Last Modified: 29 Dec 2011 11:41

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