Sood, A and Ahuja, Paramvir Singh and Sharma, Madhu and Sharma, O P and Godbole, S (2003) In vitro protocols and field performance of elites of an important bamboo Dendrocalamus hamiltonii Nees et Arn. Ex Munro. Plant Cell,Tissue And Organ Culture, 71 (1). pp. 55-63. ISSN 0167-6857

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Seedling raised elites of Dendrocalamus hamiltonii Nees et Arn. Ex Munro were chosen as the source of nodal explants from precocious branches. While axillary bud break was accomplished in hormone free 1/2MS medium containing sucrose (3%, w/v), BA supplementation was required for shoot proliferation. A variety of hormonal combinations induced rooting in clumps of shoots. Somatic embryogenesis was also obtained in callus cultures raised in 2,4-D supplemented MS medium and plantlets derived from somatic embryos were hardened for field transfer. Comparative growth performances of plants raised from nodal cuttings of field-grown plants, those from single node cuttings of precocious branches and from somatic embryos indicated that growth performance of the tissue culture raised plants was relatively better than those from nodal cuttings. Improved protocols for efficient micropropagation are visualized to provide an impetus to raising of bamboo nurseries of elite genotypes in bamboo growing areas of western Himalayas.

Item Type: Article
Uncontrolled Keywords: micropropagation; elite bamboo; transplantation; field performance
Subjects: Nutraceuticals
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 29 Dec 2011 06:21
Last Modified: 29 Dec 2011 06:21

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