Vyas, P and Rahi, P and Gulati, Arvind (2009) Stress Tolerance and Genetic Variability of Phosphate-solubilizing Fluorescent Pseudomonas from the Cold Deserts of the Trans-Himalayas. Microbial Ecology , 58 (2). pp. 425-434. ISSN 0095-3628

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Abstract

Nineteen efficient phosphate-solubilizing fluorescent Pseudomonas from the cold deserts of the trans-Himalayas were screened for stress tolerance against temperature, alkalinity, salinity, calcium salts, and desiccation. Phylogenetic analysis based on 16S rRNA gene sequencing placed these bacteria under three groups with fourteen strains in Group I including Pseudomonas trivialis and P. poae, two strains in Group II together with Pseudomonas kilonensis and P. corrugata, and three strains in Group III along with Pseudomonas jessenii and P. moraviensis. Genetic diversity assessed by ERIC and BOX-PCR revealed variability among strains belonging to the same phylogenetic groups. Cluster analysis based on the growth characteristics under regimes of different stress levels placed the strains into three distinct clusters displaying no correlation to their phylogenetic groups. Stress-tolerant strains differed in the level of decline in phosphate solubilization under increasing intensity of various stress parameters. The highest decrease occurred with 5% CaCO(3,) followed by 2.5% CaCO(3), pH 11, 5% NaCl, temperature of 37A degrees C, 40% PEG, 5% CaSO(4), 2.5% NaCl, 2.5% CaSO(4), pH 9 and temperature of 15A degrees C. Two strains belonging to Phylogenetic Group I exhibited higher phosphate solubilization at lower temperature. The results revealed that stress-tolerance ability was not limited to any particular phylogenetic group. Knowledge about the genetic variants of phosphate-solubilizing fluorescent Pseudomonas with potential for tolerance to desiccation, alkalinity, temperature, and salinity could be useful in understanding their ecological role under stressful environments of low phosphate availability.

Item Type:	Article
Uncontrolled Keywords:	Sinorhizobium-Meliloti; Temperature-Tolerant; Alkaline Soils; Bacteria; Rhizosphere; Diversity; Growth; Salt; Strain; Plant
Subjects:	Microbiology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	29 Dec 2011 04:43
Last Modified:	29 Dec 2011 04:43
URI:	http://ihbt.csircentral.net/id/eprint/348

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