Sharma, N and Sharma, U K and Ghosh, P and Sinha, Arun K and Sood , S and Gulati, Arvind (2009) Microwave-Assisted Efficient Extraction and Stability of Juglone in Different Solvents from Juglans regia: Quantification of Six Phenolic Constituents by Validated RP-HPLC and Evaluation of Antimicrobial Activity. Analytical Letters, 42. pp. 2592-2609.

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In the present study, microwave-assisted extraction was compared with conventional approaches for the efficient extraction of juglone and other phenolics from Juglans regia bark. The effect of different solvents was also studied and ethyl acetate was found to be a better solvent in terms of juglone yield and stability. Further, a simple and fast RP-HPLC method was developed and validated for the determination of juglone and other bioactive phenolics like gallic acid, caffeic acid, quercetin, myricetin, and quercitrin in these extracts. In addition, the extracts were tested for antimicrobial activity against 16 microorganisms where all the extracts showed broad spectrum activity.

Item Type: Article
Uncontrolled Keywords: Antimicrobial activity, juglone, Juglans regia, microwave assisted extraction, phenolic compounds, RP-HPLC, stability
Subjects: Microbiology
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 02 Jan 2012 10:06
Last Modified: 02 Jan 2012 10:06

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