Jaitak, V and Kaul , V K and Kumar, Neeraj and Singh , Bikram and ---, Bandna and Savergave, L S and Jogdand, V V and Nene, S (2009) Simple and efficient enzymatic transglycosylation of stevioside by beta-cyclodextrin glucanotransferase from Bacillus firmus. Biotechnology Letters, 31 (9). pp. 1415-20. ISSN 0141-5492

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Abstract

Stevioside was subjected to 1,4-intermolecular transglycosylation using beta-cyclodextrin glucanotransferase (beta-CGtase) produced from an alkalophilic strain of Bacillus firmus. The reaction was carried out by traditional, ultrasound-assisted and microwave-assisted techniques. Reaction under microwave conditions was faster and was completed in 1 min yielding two 1,4 transglycosylated products, 4'-O-alpha-d-glycosyl stevioside (I) and 4''-O-alpha-d-maltosyl stevioside (II) in 66% and 24%, respectively. The optimum transglycosylation occurred by using stevioside (1.24 mmol), beta-CD (1.76 mmol) and beta-CGtase (2 U/g) under microwave assisted reaction (MAR) in 5 ml sodium phosphate buffer (pH 7) at 50A degrees C and 80 W power. MAR is therefore potentially a useful and economical method for faster transglycosylation of stevioside.

Item Type:	Article
Uncontrolled Keywords:	Beta-Cyclodextrin Glucanotransferase; Beta-Cyclodextrin; Microwave-Assisted Reaction; Stevia Rebaudiana; Stevioside; Transglycosylation
Subjects:	Plant Biotechnology Natural Product Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	28 Dec 2011 10:09
Last Modified:	02 Jan 2013 12:33
URI:	http://ihbt.csircentral.net/id/eprint/334

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