Bhandari, P and Kumar, Neeraj and Singh , Bikram and Gupta , A P and Kaul , V K and Ahuja, Paramvir Singh (2009) Stability-Indicating LC-PDA Method for Determination of Picrosides in Hepatoprotective Indian Herbal Preparations of Picrorhiza kurroa. Chromatographia, 69 (3-4). pp. 221-227. ISSN 0009-5893

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A liquid chromatography method was developed and validated for the determination of picroside-I and picroside-II in herbal preparations containing Picrorhiza kurroa as one of the ingradients. Resolution of picrosides was achieved on a reversed phase (C-18) endcapped bidentate column by using a mobile phase of acetonitrile: water (25:75 v/v). The detection of picrosides was carried out at 270 nm. The method was validated for precision, accuracy and robustness according to the International Conference on Harmonization (ICH) guidelines and is applicable for the quality control of preparations containing P. kurroa. Analysis of samples in forced degradation proved it to be applicable for stability evaluation. The linear regression analysis data showed good linear relationship (r (2) = 0.9999 +/- A 0.0010 for picroside-I and 0.9997 +/- A 0.0012 for picroside-II) in the concentration range of 0.4-4.0 mu g. The limit of detection and quantification for picroside-I and picroside-II were recorded to be 28.1 and 73.1 ng and 85.2 and 221.5 ng, respectively. Satisfactory recovery results were observed from the herbal preparations (97.5-100.5%). Intra- and inter- day precision of the method was acceptable, with relative standard deviation (%RSD) values in the range of 0.04-1.16% and 0.03-0.27%, respectively.

Item Type: Article
Uncontrolled Keywords: Column liquid chromatography; Stability evaluation; Herbal preparations; Picroside-I and picroside-II; Picrorhiza kurroa; Liquid-Chromatography; Mass-Spectrometry; Medicinal-Plants; Assay; Stress; Kutkin; Benth
Subjects: Natural Product Chemistry
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 28 Dec 2011 06:30
Last Modified: 06 Feb 2012 06:49

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