Sharma, Madhu and Pati, P K and Ahuja, Paramvir Singh (2005) Extra thin alginate film: an efficient technique for protoplast culture. Protoplasma, 226 (3-4). pp. 217-221.

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This paper reports an efficient protoplast culture technique, the “extra thin alginate film’’ technique. The development of this improved method of protoplast culture was an outcome of an assessment of the efficiency and shortcomings of various protoplast culture techniques. The efficiency of this technique was evaluated with two model plant systems, viz., Nicotiana tabacum and Lotus corniculatus, and a comparison was made with the “thin alginate layer’’ technique, another efficient protoplast culture system. Results indicate that the culture technique with extra thin alginate film is as efficient as the technique with thin alginate layer, with many additional advantages. The present innovation overcomes most of the limitations of protoplast culture techniques described so far and can now be applied to a wide variety of crops to check its general applicability.

Item Type: Article
Uncontrolled Keywords: Protoplast; Thin-alginate-layer technique; Extra-thin alginate- film technique; Nicotiana tabacum; Lotus corniculatus.
Subjects: Plant sciences
Plant Biotechnology
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 18 Nov 2011 07:32
Last Modified: 10 Feb 2012 07:38

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