Bhandari, P and Singh , Bikram and Gupta , A P and Kaul , V K (2005) Simultaneous densitometric determination of artemisinin, artemisinic acid and arteannuin-B in Artemisia annua using reversed-phase thin layer chromatography. Journal of Separation Science, 28. pp. 2288-2292.

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Abstract

A rapid and simple RP-TLC method for simultaneous quantification of pharmacologically important sesquiterpene artemisinin (AM) together with its precursors arteannuin- B (AB) and artemisinic acid (AA) in the inflorescence part of Artemisia annua plant has been developed. The RP-TLC of sesquiterpenes was performed on RP-18 F254 S thin-layer chromatographic plates by developing in mobile phase, containing 0.2% TFA in water/ACN (35 : 65, v/v). The densitometric determination of AM, AB and AA was carried out after derivatization with anisaldehyde reagent at 426 nm in absorption-reflectance mode.

Item Type:	Article
Uncontrolled Keywords:	Arteannuin-B; Artemisia annua; Artemisinic acid; Artemisinin; Densitometry; RPTLC;
Subjects:	Natural Product Chemistry Information and Science Management
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	29 Dec 2011 11:27
Last Modified:	13 Feb 2012 06:06
URI:	http://ihbt.csircentral.net/id/eprint/256

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