Verma, N and Singh, L and Singh, A K and Kulshrestha, S and Hallan, Vipin and Raikhy, G and Raja, Ram and Zaidi, A A (2005) Ornithogalum: a new host of Cucumber mosaic virus (CMV) from India. Plant Pathology, 54. p. 256.

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Abstract

Ornithogalum thyrsoides (family Hyacinthaceae) is an ornamental cut flower crop which has a long vase life and is resistant to extreme temperatures. Plants have been reported to be infected with Ornithogalum mosaic virus (OMV) and Tobacco necrosis virus (TNV) (Burger & Wechmar, 1995). Ornithogalum plants growing in a floriculture field at the Institute of Himalayan Bioresource Technology, Palampur, were found exhibiting severe mosaic on the leaves. The causal virus was found to be transmitted mechanically, causing local lesions on Chenopodium album and C. amaranticolor , and systemic mosaic on Nicotiana clevelandii , N. glutinosa , N. megalosiphon and N. tabacum cv. White Burley. In ELISA, the virus reacted with Cucumber mosaic virus (CMV)-specific antibodies procured from Agdia (USA). Leaf dip preparations from virus-infected plants in electron microscopy revealed the presence of ∼ 29 nm isometric particles. In immunoelectron microscopy (IEM), the virus was readily trapped and decorated with CMV-specific antibodies. Total RNA was isolated from healthy and naturally infected ornithogalum, as well as from inoculated plants, using RNAqueous (Ambion, USA) as per the manufacturer’s instructions. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using the CPTALL primer pair (Choi et al ., 1999). Amplicons of expected size ( ∼ 950 bp) were obtained in virus-infected and inoculated plants. No amplification was observed in healthy ornithogalum plants. PCR-amplified products were cloned and sequenced (AJ745092). BLAST searches revealed the nucleotide and the deduced amino acid sequence showing 97 and 93–95% similarity with the available sequences of CMV subgroup II, respectively, confirming the association of CMV with the severe mosaic on ornithogalum. This is the first definitive report of CMV infecting ornithogalum.

Item Type:	Article
Subjects:	Plant viruses
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	02 Jan 2012 10:16
Last Modified:	02 Jan 2012 10:16
URI:	http://ihbt.csircentral.net/id/eprint/231

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