Singh, K and Paul, A and Kumar, Sanjay and Ahuja, Paramvir Singh (2009) Cloning and differential expression of QM like protein homologue from tea [Camellia sinensis (L.) O. Kuntze]. Molecular Biology Reports, 36. pp. 921-927.

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Abstract

The QM like protein gene encodes for ribosomal protein L10, which is implicated in tumor suppression, transcription factor regulation, and ribosome stability in yeast and mammals. Present study describes cloning of a full-length QM cDNA (CsQM) from tea leaves using differential display of mRNA followed by rapid amplification of cDNA ends. Expression of CsQM was studied in leaves of different stages of development and under various external cues. CsQM contained an open reading frame of 651 bases, encoding 216 amino acids. CsQM shared 71–87% and 85–91% identity at nucleotide and amino acid sequences, respectively with QM genes isolated from other plant species. During active-growth period of tea, higher expression was observed in apical buds that decreased gradually with increasing age of the leaf. During dormancy season, the expression of CsQM gene was severely down-regulated in all the leaves studied. CsQM transcript was found to be down regulated in response to drought stress and abscisic acid treatment but up-regulated by gibberellic acid treatment. A positive association of CsQM transcript abundance with active cellular growth suggested its role in plant growth and development

Item Type:	Article
Uncontrolled Keywords:	Camellia sinensis. Development. Gene expression. Stress. Tea. QM like protein
Subjects:	Plant sciences Nutraceuticals
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	29 Dec 2011 10:09
Last Modified:	06 Feb 2012 06:22
URI:	http://ihbt.csircentral.net/id/eprint/223

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