Singh, N and Singh , Bikram and Gupta , A P and Kaul , V K (2005) Quantification of Picroside-I and Picroside-II in picrorhiza kurroa by HPTLC. Journal of Chromatography and Related Technologies, 28 (11). pp. 1679-1691. ISSN 1082-6076

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A new high performance thin layer chromatography (HPTLC) method for the simultaneous quantification of picroside-I and picroside-II in P. kurroa is described. Separation of picroside-I and picroside-II was achieved by mobile phase of CHCl3:MeOH (82:18, v/v) on precoated silica gel 60 F254 aluminum plate. The densitometric determination of picrosides was carried out at 290 nm, in absorptionreflection mode. The calibration curves were linear in the range of (2–5mg). The method is simple, specific, rapid, and reliable for simultaneous determination of P-I and P-II in P. kurroa. The proposed method was applied for accurate quantification of large number of samples collected from different altitudes of western Himalaya.

Item Type: Article
Uncontrolled Keywords: Picroside-I, Picroside-II, Picrorhiza kurroa, HPTLC; Thin-Layer-Chromatography; Liquid-Chromatography; Mass-Spectrometry
Subjects: Natural Product Chemistry
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 27 Dec 2011 10:14
Last Modified: 27 Dec 2011 10:14

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