Raja, Ram and Verma, N and Singh, L and Hallan, Vipin and Singh, A K and Zaidi, A A (2005) Indexing and production of virus-free chrysanthemums. Biologia Plantarum, 49. pp. 149-152.

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Abstract

Chrysanthemum morifolium cv. Regol Time plants were found to be infected with Chrysanthemum B carlavirus (CVB). They were made CVB-free by using meristem tip culture, chemotherapy and thermotherapy. The plants were indexed by biological assay, double antibody sandwitch enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). The maximum number of virus-free plants (26.7 %, as indexed by RT-PCR) was obtained with 2-thiouracil at 0.04 g dm-3 concentration. Only 12 % plants were found to be virus-free, after being kept at 38 °C for 30 d. For indexing CVB in chrysanthemums, RT-PCR was found to be the most reliable method.

Item Type:	Article
Uncontrolled Keywords:	antiviral compounds, Chrysanthemum B carlavirus, Chrysanthemum morifolium, DAS-ELISA, in vitro production, RT-PCR.
Subjects:	Plant sciences Plant viruses
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	02 Jan 2012 09:59
Last Modified:	02 Jan 2012 09:59
URI:	http://ihbt.csircentral.net/id/eprint/198

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