Verma, N and Sharma, A and Hallan, Vipin and Raja, Ram (2003) Detection, identification and incidence of Chrysanthemum B carlavirus in chrysanthemum in India. Crop Protection, 22. pp. 425-429.

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During a survey of chrysanthemum cvs. grown in Himachal Pradesh. (India) a variety of symptoms were seen. The disease incidence ranged between 40.62% and 94.66%. Out of 36 cvs. surveyed 7 were found to be negative for CBV in DAS-ELISA. The virus has a very narrow host range infecting only N. clevelandii, N. glutinosa, N. rustica, Petunia hybrida and Vicia faba. In cytopathology infected cells showed absence of specific inclusion body (ies), presence of virus particles in large numbers along the outer envelope of chloroplast with abnormalities like electron dense matrix, loss of chloroplast envelope and extensive invegination of cytoplasm. In immune electron microscopy there was heavy decoration and trapping of virus particle with the antiserum specific to CBV. The particle length was ca. 680�12 nm. The causal virus was found to be Chrysanthemum B carlavirus on the basis of host range, DAS-ELISA, immune electron microscopy and cytopathology. This may have practical benefits for producing virus-free stocks of chrysanthemum for growers

Item Type: Article
Uncontrolled Keywords: Cytopathology chrysanthemum carlavirus
Subjects: Plant viruses
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 29 Dec 2011 07:33
Last Modified: 07 Feb 2012 11:49

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