Verma, N and Mehra, A and Singh, L and Hallan, Vipin and Singh, A K and Jabeen, N and Singh, M K and Raja, Ram and Zaidi, A A (2007) Screening for viruses infecting chrysanthemum cultivars in India. Scientia Horticulture, 111 (3). pp. 260-265. ISSN 0304-4238

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Abstract

Screening of chrysanthemum cultivars was carried out using enzyme linked immunosorbent assay (ELISA), northern hybridization and reverse transcription polymerase chain reaction (RT-PCR) for Chrysanthemum B (CVB), Cucumber mosaic (CMV), Tomato aspermy (TAV), tospo- and potyviruses. By hybridization and RT-PCR, 48.7%, 42.5%, 26.2%, 12.5% and 6.25% cultivars were found to be infected with CVB, CMV, TAV, tospo- and potyviruses, respectively out of 80 cultivars tested. CVB was the most prevalent virus while infections by poty- and tospoviruses were found less frequently. The percentage of the single virus infection in chrysanthemum cultivars under study was the highest (35%) followed by double (26.25%), triple (8.75%) and for tetra and pentavirus infection it was 2.5%. Nucleic acid based detection techniques were found to be more reliable and sensitive than ELISA.

Item Type:	Article
Uncontrolled Keywords:	Chrysanthemum virus B; Cucumber mosaic virus; Tomato aspermy virus; Potyvirus; Tospovirus
Subjects:	Plant sciences
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	27 Dec 2011 11:53
Last Modified:	27 Dec 2011 11:53
URI:	http://ihbt.csircentral.net/id/eprint/185

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