Negi, A and Rana, T and Kumar, Y and Hallan, Vipin and Raja, Ram and Zaidi, A A (2010) Analysis of the Coat Protein Gene of Indian Strain of Apple Stem Grooving Virus. Journal of Plant Biochemistry and Biotechnology, 19 (1). pp. 91-94.

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Abstract

A survey was undertaken in the temperate fruit growing regions of Himachal Pradesh (HP) and Jammu & Kashmir (J&K). Apple stem grooving virus (ASGV), a Capillovirus, was detected in different cultivars of apple, nectarines, plum, cherry, quince and apricot by double antibody sandwich ELISA (DAS-ELISA). The coat protein (CP) gene sequence of an amplicon produced by RT-PCR, confirmed the association of ASGV in apple cultivar Starkrimson, collected from Himachal Pradesh. The CP of Indian ASGV isolate shared 100 % sequence identity with a Brazilian isolate (AF438409). Sequence analysis by Recombination Detection Program (RDP2) indicated no recombination event for the Indian isolate. However, recombination was detected in Chinese, Korean and Citrus tatter leaf virus-Taiwan (CTLV) strains of ASGV. The study describes first report of ASGV infection in India and characterization of its CP gene.

Item Type:	Article
Uncontrolled Keywords:	ASGV, CTLV, CP, apple, cherry, nectarine, plum, quince apple, ELISA, RT-PCR.
Subjects:	Plant sciences Plant Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	02 Jan 2012 09:53
Last Modified:	02 Jan 2012 09:53
URI:	http://ihbt.csircentral.net/id/eprint/156

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