Bhatti, S and Jha, Gopaljee (2010) Current trends and future prospects of biotechnological interventions through tissue culture in apple. Plant Cell Reports, 29 (11). pp. 1215-1225. ISSN 0721-7714

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Apple (Malus domestica Borkh.), which is a widely cultivated, important economic fruit crop with nutritive and medicinal importance, has emerged as a model horticultural crop in this post-genomic era. Apple cultivation is heavily dependent on climatic condition and is susceptible to several diseases caused by fungi, bacteria, viruses, insects, etc. Extensive research work has been carried out to standardize tissue culture protocols and utilize them in apple improvement. We review the in vitro shoot multiplication, rooting, transformation and regeneration methodologies in apple and tabulate various such protocols for easy reference. The utility and limitation of transgenesis in apple improvement have also been summarized. The concepts of marker-free plants, use of nonantibiotic resistance selectable markers, and cisgenic and intragenic approaches are highlighted. Furthermore, the limitations, current trends and future prospects of tissue culture-mediated biotechnological interventions in apple improvement are discussed.

Item Type: Article
Uncontrolled Keywords: Micropropagation. Disease resistance. Apple scab. Fruit quality. Shelf life. Transgenic. Cisgenic. Intragenic
Subjects: Plant Biotechnology
Depositing User: Dr. Aparna Maitra Pati
Date Deposited: 27 Dec 2011 10:44
Last Modified: 27 Dec 2011 10:44

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