Hossain, M M and Sharma, Madhu and Pathak, Promila (2012) In vitro propagation of Dendrobium aphyllum (Orchidaceae)—seed germination to flowering. Journal of Plant Biochemistry Biotechnology.

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Abstract

This communication describes asymbiotic seed germination, protocorm development, micropropagation and flowering in in vitro and hardened seedlings of Dendrobium aphyllum (Roxb.) C.E.C. Fischer. Effects of four culture media viz., Murashige and Skoog (MS); Phytamax (Sigma Chemical Co. USA; PM); Mitra et al. (M) and Knudson ‘C’ (KC), 6-benzylaminopurine (BAP) and 2,4- dichlorophenoxyacetic acid (2,4-D), peptone and activated charcoal were studied on seed germination and protocorm development. Maximum germination (97 %) was recorded in PM basal medium. Peptone (2.0 gl−1) remarkably enhanced germination percentage (100 %), vigorous growth, high survival and subsequent development of protocorms, while in activated charcoal the response was not encouraging. BAP improved germination percentage, however, 2,4-D showed noticeably low seed germination. The morphogenetic response of protocorms and nodal segments of in vitro raised seedlings varied depending on type of explants and concentrations and combinations of plant growth regulators used. Stout root system was induced in 1/2PM + 0.5 mgl−1 IAA. Approximately 10 % of the in vitro raised plants (4– 5 cm) with 3–4 leaves flowered in vitro irrespective of flowering season. The well-rooted plants showed 80 % survival under green house conditions and flowering was noticed after 5–6 months in 10 % of hardened plants.

Item Type:	Article
Uncontrolled Keywords:	Seed germination . Protocorm development . PLBs . Micropropagation
Subjects:	Plant sciences Plant Biotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	05 Apr 2013 12:34
Last Modified:	05 Apr 2013 12:34
URI:	http://ihbt.csircentral.net/id/eprint/1353

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