Chandel, Madhu and Sharma, Upendra and Kumar, Neeraj and Singh , Bikram (2012) Antioxidant activity and identification of bioactive compounds from leaves of Anthocephalus cadamba by ultra-performance liquid chromatography/electrospray ionization quadrupole time of flight mass spectrometry. Asian Pacific Journal of Tropical Medicine. pp. 977-985.

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Abstract

Objective: To evaluate the antioxidant potential of different extract/fractions of Anthocephalus cadamba (A. cadamba) (Roxb.) Miq. (Rubiaceae) and study the tentative identification of their active constituents. Methods: The extract/fractions were screened for antioxidant activity using various in vitro assays viz. DPPH assay, ABTS assay, superoxide anion radical scavenging assay, wreadsu cdientge rpmoiwneerd a bssya cy oalnordi mpleatsrmici dm DetNhAod n. icAkni nugl tarsas-apye. rTfoortaml apnhceen oLlCic- ecloenctternots porf aeyx-trqaucat/dfrraucptoiolen-s tfirmacet ioonf sf liogf hAt . mcaadssa mspbeac. trRoemseutlrtys :m Tehteh oedth wyla sa cuesteadte tfor aacntiaolny swe atsh efo aucntdiv teo cboen mstiotsute anctsti voef ferxatcrtaicotn/ (iEn AaAllC t hfrea catsisoany)s w aass c2o1m.2p4a red to other extract/fractions. The IC50 value of ethyl acetate fraction µg/mL, 1.12 ug/mL, 9.68 µg/mL and 57.81 µg/mL in DPPH assay, AfrBacTtSio ansss aayl,s ore sdhuocwinegd ptohwe epr oatsesnatyia al ntdo spurpoteercotx itdhee spclaavsemnigdi ng assay respectively. All the extract/ hydroxyl radicals generated by DNA (pBR322) against the attack of Fenton’s reagent. The bioactive compounds were identified by UPLC-ESI-QTOF-MS, by comparing the mass and λmax with literature values. Conclusions: The pthoatet nthtieayl mofa tyh eb ee xutsreafcutl/ ftrhaecrtaiopnesu ttioc sacgaevnetnsg feo rd tirfefearteinngt frraedei craald-icreallast eind dpiaftfheroelongt iscy dstaemmasg ein.dicated

Item Type:	Article
Uncontrolled Keywords:	Anthocephalus cadamba AUnPtLioCx-idEaSnIt- aQcTtiOviFty-MS
Subjects:	Natural Product Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	05 Apr 2013 11:07
Last Modified:	05 Apr 2013 11:07
URI:	http://ihbt.csircentral.net/id/eprint/1349

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