Bhardwaj, Jyoti and Mahajan, Monika and Yadav , S K (2013) Comparative Analysis of DNA Methylation Polymorphism in Drought Sensitive (HPKC2) and Tolerant (HPK4) Genotypes of Horse Gram (Macrotyloma uniflorum). journal of Biochemical genetics.

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Abstract

DNA methylation is known as an epigenetic modification that affects gene expression in plants. Variation in CpG methylation behavior was studied in two natural horse gram (Macrotyloma uniflorum [Lam.] Verdc.) genotypes, HPKC2 (drought-sensitive) and HPK4 (drought-tolerant). The methylation pattern in both genotypes was studied through methylation-sensitive amplified polymorphism. The results revealed that methylation was higher in HPKC2 (10.1%) than in HPK4 (8.6%). Sequencing demonstrated sequence homology with the DRE binding factor (cbf1), the POZ/BTB protein, and the Ty1-copia retrotransposon among some of the polymorphic fragments showing alteration in methylation behavior. Differences in DNA methylation patterns could explain the differential drought tolerance and the epigenetic signature of these two horse gram genotypes.

Item Type:	Article
Uncontrolled Keywords:	Drought stress; Epigenetics; Horse gram; Methylation-sensitive amplified polymorphism (MSAP)
Subjects:	Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	03 Apr 2013 06:45
Last Modified:	03 Apr 2013 06:45
URI:	http://ihbt.csircentral.net/id/eprint/1312

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