Sharma, U and Bhandari, P and Kumar, Neeraj and Singh , Bikram (2010) Simultaneous Determination of Ten Sugars in Tinospora cordifolia by Ultrasonic Assisted Extraction and LC-ELSD. Chromatographia, 71 (7-8). pp. 633-638. ISSN 0009-5893

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Abstract

Sugars present in medicinal plants are known for protecting and stimulating the immune system against various biological disorders. Tinospora cordifolia is a reputed Indian herb used for immunity enhancing which is mainly attributed to saccharides. In the present study, a simple, sensitive, and reliable liquid chromatography method based on ultrasonic assisted extraction and evaporative light scattering detection was developed for simultaneous determination of ten sugars comprising of monosaccharides (L-(+)-rhamnose, D-(+)-xylose, D-(-)-arabinose, b-D-(+)-glucose), disaccharides (sucrose, D-(+)-cellobiose, a-lactose), alditols (xylitol, D-(+)-mannitol) and a polyalcohol (myo-inositol) in T. cordifolia. The separation was achieved on Zorbax-NH2 column (250 mm 9 4.6 mm, 5 lm) in gradient elution of acetonitrile: water as mobile phase with flow rate of 0.5 mL min-1. The drift tube temperature and nitrogen flow-rate were optimized at 70 �C and 2.0 standard litres per minute, respectively. The method was validated for linearity, accuracy, precision, limits of detection and quantification. The calibration equation revealed a good linear relationship (r2 = 0.959–0.999). The sufficient recovery was observed in the range of 94.1–99.9%. The method showed good reproducibility with intra- and inter-day precision of <0.99 and 0.97% (RSD), respectively. The detection and quantification limits for the compounds were in the range of 8.32–44.29 and 25.23–134.20 lg mL-1, respectively.

Item Type:	Article
Uncontrolled Keywords:	Column liquid chromatography; Ultrasonic assisted extraction; Evaporate light scattering detection; Sugars; Tinospora cordifolia.
Subjects:	Natural Product Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	27 Dec 2011 10:25
Last Modified:	27 Dec 2011 10:25
URI:	http://ihbt.csircentral.net/id/eprint/130

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