Sherpa, A R and Hallan, Vipin and Zaidi , A A (2012) In Vitro Expression and Production of Antibody Against Cymbidium mosaic virus Coat Protein. Indian Journal of Virology, 23 (1). pp. 46-49.

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Abstract

Polyclonal rabbit antisera were produced using coat protein of Cymbidium mosaic virus (CymMV) Indian isolate expressed in E. coli as GST fusion. The expressed protein was purified by GST-fusion protein purification kit for use as an immunogen in rabbits. Antisera prepared in this manner reacted in double antibody sandwich enzymelinked immunosorbent assay (DAS ELISA) with extract from CymMV-infected tissue. The results indicate that polyclonal antisera prepared from expressed CymMV coat proteins were useful for the detection of CymMV in an array of assays. The detection system developed is highly effective for detection of Indian strain of the virus in comparison to kits available in the international market.

Item Type:	Article
Uncontrolled Keywords:	CymMV � ELISA � In vitro � Expression � Orchidaceae � CP
Subjects:	Plant sciences Plant viruses
Divisions:	UNSPECIFIED
Depositing User:	Dr. Aparna Maitra Pati
Date Deposited:	31 Jan 2013 12:53
Last Modified:	31 Jan 2013 12:53
URI:	http://ihbt.csircentral.net/id/eprint/1295

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